Someone should start with a reasonable estimate of how many galleys Venice can actually deploy before going into proclamations of the end of its naval power. We know Venice deployed 33 galleys against the Cretan revolt. Presumably they had additional naval deployments at the time. In the next war with Genoa they had 48 galleys at Chioggia after losing 15 more and over 3,000 men at Pola. Which means a total force around 63 galleys after losing Dalmatia and fighting a second war with Hungary.
A few arguments on the general estimation. In the 1350s Crete provided 4 galleys each year and in 1362 the Cretans were ordered to arm 6. At the same time, they seem to have provided a lot of crossbowmen, with 500 being a number I have seen. These are more crossbowmen that cretan 4-6 galleys need. That means that the crossbowmen for a few other galleys were Cretans. During the War of Choggia, the hulls of 5 light galleys were stored in Modon. I think it would be unlikely that the two small cities could man these ships and at the same time garrison themselves. After all, their population was mixed and included Greeks and Jews who were not drafted - in contrast to Crete where Greeks provided crews. I would think that the vast majority of the crews were Latins from the Peloponnese and the Ionian Islands. And then there are the few galleys from Cyclades and Negroponte. Overall, if there is a war, these sources of ships and naval manpower will be tied down, in revolt or not exist at all (Latin statelets and Cyclades).
The original force that sailed with Pisani and defeated the Genoese off Anzio consisted of 15 galleys. After his victory took his fleet east to Cyprus to raid Genoese shipping, met up with additional galleys from the venetian colonies of the Aegean and wintered in Pola. It seems that in Pola they had around 20-25 galleys, so the Aegean squadron must have been around 6-10 galleys, consisted with the above figures. It is difficult to properly calculate the number of Pisani's galleys because part of his crews died during the winter with many sick. Some galleys were on land without crews and at least two had only half their normal complement. He received some replacements from Venice but even so it seems that only 16 of his galleys had full crews.
Then, we have the fleet of Carlo Zeno. He left Venice with 5 galleys to harry the genoese trade. Then he received another 6 galleys and returned home to participate in Chioggia with ... 14. I think it is plausible that the additional 3 galleys were from the venetian colonies.
Overall, it seems that the Venetians started the War of Chioggia with 26 galleys from the metropole and 9-13 from its colonies.
After the defeat at Pola, Pisani returned to Venice with 6 galleys. After a political debacle when the people demanded that he would re-appointed admiral, there was a total mobilization. They managed to build 40 galleys but they were able to man only 34 in total, the 6 Pola survivors included. By giving a spear or an oar to every teenager and greybeard, they managed to man 28 additional galleys. Although it should be noted that they must have manned also light craft. After all, in the afternath of Chioggia when they sent a fleet against Istria, it included light craft as well.
The Venetians in 1379 could produce a fleet of 54 galleys, but only with total mobilization to save their city. It would be neither prudent nor sustainable to fight a protracted naval war with untrained crews.
Back in 1363, the impact of the Plague would be more noticeable but they would also have access to dalmatian naval power. Back in 1350 the Dalmatians could man perhaps 10 galleys. Most likely fewer, since of the 35-strong fleet, 25 were from the metropole and the rest from Dalmatia and the rest of the colonies. But if the Hungarians are invading, I could see the dalmatian cities providing only token resistance as in OTL 1357 - they were as rebelious as the Cretans, or more. And the cities that would resist, they would need their fighting men on theirs walls.
